Purpose: Portal vein transfusion (PVT) has been known to induce immunosuppression or tolerance and Kupffer cell was identified to play an important role in the phenomenon. The purposes of this study were investigating PVT effect on gene
regulation in Kupffer cells and subsequent change in serum cytokine.
Methods: For investigating the effect of PVT, Kupffer cells were isolated from the mice (BalbC) of six groups; 1 hour sham operation (S), 1 hour portal vein saline injection (PVS), 1 hour PVT, 24 hour S, 24 hour PVS, and 24 hour PVT
groups.
Each
group was composed of 3 mice. Total RNAs isolated from Kupffer cells were subjected to RT-PCR differential display. The bands of 24 hour group showing increased expression was cloned for the sequencing analysis.
Results: Macrophage inflammatory protein 1 alpha (MIP-1α) was identified from the bands of increased expression. In PVT groups, increased expression of MIP-1α mRNA in Kupffer cells coincided with elevated serum level of MIP-1α.
Conclusion: MIP-1α may be one of the important cytokines involved in PVT induced immunosuppression or tolerance.
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